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PCR is a sensitive and effective method for amplifying a single copy of a target DNA sequence to millions of copies in a short time frame. Therefore, plastic consumables for PCR reactions must be free of contaminants and inhibitors, while having high quality that can guarantee the best PCR effect. PCR plastic consumables are available in a variety of sizes and formats, and knowing the appropriate characteristics of the products will help you in selecting the right plastic consumables for optimal PCR and qPCR data.
Composition and Characteristics of PCR consumables
PCR consumables are typically made of polypropylene, which is inert enough to withstand rapid temperature changes in the course of thermal cycling and minimize the uptake of reactive substances to ensure optimal PCR results.To further ensure batch-to-batch consistency in purity and biocompatibility, medical-grade, high-quality polypropylene raw materials should be used during production and manufactured in a Class 100,000 cleanroom. The product must be free of nuclease and DNA contamination to avoid interfering with the effect of DNA amplification experiments.
PCR plates and PCR tubes are generally available in transparent and white.
The PCR Plate "skirt" is around the board. The skirt provides better stability for the pipetting process when the reaction system is constructed, and provide better mechanical strength during the automatic mechanical treatment. PCR plate can be divided into no skirt, half skirt and full skirt.
PCR tube is available in single and 8-strips tube, which are more suitable for low to medium throughput PCR/qPCR experiments. The flat cover is designed to facilitate writing, and the high fidelity transmission of fluorescence signal can be better realized by qPCR.
The tube cover and sealing film must completely seal the tube and plate to prevent evaporation of the sample during the thermal cycle. A tight seal can be realized by using a film scraper and a press tool.
The experimental flux of the PCR / qPCR assays may determine which type of plastic consumables should be used for the best treatment effect. For low-to-moderate throughput applications, tubes are generally more suitable, while plates are more desirable for medium-to-high throughput experimental. Plates are also designed to consider the flexibility of flux, which can be divided into a single strip.
In conclusion, as an important part of PCR system construction, plastic consumables are crucial for the success of experiments and data collection, especially in medium-to-high throughput workflow applications.
As a chinese supplier of automated plastic consumables, Cotaus provides pipette tips, nucleic acid, protein analysis, cell culture, sample storage,sealing, chromatography, etc.
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