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Do you know what is PCR and what is PCR plate? As a professional medical consumable supplier, we will take you to understand the explanation of these professional terms.
PCR: PCR (polymerase chain reaction) uses DNA to become single-stranded when it is denatured at a high temperature of 95°C in vitro. At low temperatures (usually around 60°C), the primers and single-strands are combined according to the principle of complementary base pairing. Then adjust the temperature to the optimal reaction temperature for DNA polymerase (about 72°C), and the DNA polymerase will synthesize complementary strands along the direction from phosphoric acid to five carbon sugars (5°-3°). The PCR machine based on polymerase is actually a temperature control device, which can well control the denaturation temperature, renaturation temperature, and elongation temperature.
PCR plate: In Polymerase Chain Reaction (PCR), it is mainly used as a carrier for primers involved in the amplification reaction, Taq DNA polymerase, dNTP, template nucleic acid, Mg, buffer, etc.
Its own material is mainly polypropylene (PP) nowadays, which can better adapt to repeated high and low temperature settings in the PCR reaction process, and can achieve high temperature and high pressure sterilization. In order to achieve high-throughput operation in conjunction with a row gun, PCR machine, etc., 96-well or 384-well PCR plates are more commonly used. The plate shape conforms to the SBS international standard, and in order to adapt to the PCR machines of different manufacturers, according to the skirt design, it can be divided into four design modes: no skirt, half skirt, raised skirt and full skirt.
The colors are transparent and white, among which white is more suitable for the new type of real-time fluorescent quantitative PCR.
The use of PCR is widely used in genetics, biochemistry, immunity, medicine and other fields. It is not only used in basic research such as gene isolation, cloning and nucleic acid sequence analysis, but can also be used for disease diagnosis or any place with DNA and RNA. It is an experiment disposable consumables.
If you happen to looking for a professional manufacture of PCR plate, you absolutely can consider us as your first choice.
ConRem is a high-tech enterprise engaged in R&D, production and sales of high throughput automated IVD and lab consumables.
Suzhou ConRem Biomedical Technology Co., Ltd values personnel training and R&D support. We own more than 10 R&D experts whom are from fields of laboratory medicine, materials science, mechanical design, automation, etc. They all have decades theoretical and R&D practical experience in IVD Industry and focus on product development, design and mold manufacturing. Our R&D team help decompose customers’ needs into all areas of the business, improve product competitiveness, save the time and cost for customers and provide overall solutions.
If you have any need for PCR plate, please do not hesitate to place an order with us! We will be your trusted partner.